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Skin refreshing, lightening & moisturizing by Vitamin C, E, & Y-PGA Iontophoresis

Vitamin C is ascorbic acid and is naturally found in nature, while some animals have the ability to produce their own vitamin C, human beings do not produce vitamin C themselves and are totally reliant on the vitamin C they get in their diets.


Vitamin C is ascorbic acid and is naturally found in nature, while some animals have the ability to produce their own vitamin C, human beings do not produce vitamin C themselves and are totally reliant on the vitamin C they get in their diets. Vitamin C is a water-soluble vitamin and we do not have large stores of vitamin C in our body. If we don't eat vitamin C then within about six weeks we develop the signs of deficiency of vitamin C, that is scurvy. Vitamin C is normally deposited in the skin and is an essential part of the anti-oxidant brigade to protect skin against free radical assault from the atmosphere and from ultra violet light. Vitamin C plays a very important in converting inactivated vitamin E back into an active anti-oxidant form of vitamin E. This is probably the reason why vitamin C has such an important role to play in the protection of cellular membranes even though it is a water soluble product while cellular membranes are mainly composed of lipid molecules.

Vitamin C is denatured in the skin by exposure to blue light and also to ultra violet light in the group A. As with vitamin A we probably develop a chronic deficiency of vitamin C in all the areas of skin that are exposed to sunlight. Vitamin C does not seem to have any activity on DNA itself, but certainly does work on various enzymes in the body. Therefore vitamin C has both an anti-oxidant activity and a metabolic activity.

Vitamin C in photoageing:

Vitamin C plays a potent role in diminishing the effects of free radical damage and in this role can be quite effective as a protectant from ultra violet light damage. The advantage of vitamin C over a sunscreen is that vitamin C can be absorbed into the cells and is generally still present about 30-36 hours after it has been applied topically to the skin. It will, therefore, still give sun protection even though the subject may have washed their skin or gone swimming. As a result of this we can expect that vitamin C can slow down photoageing.


Vitamin C plays an essential part in the incorporation of proline into collagen and is also involved in the formation of elastin. With a deficiency of vitamin C impaired collagen is created and the skin can become more wrinkled. The replacement of vitamin C boosts the manufacture collagen and so wrinkles can become less noticeable after using vitamin C.


Vitamin C effects pigmentation in two ways: The creation of melanin is an oxidative process and so a powerful antioxidant like vitamin C could counteract the oxidative process required to create melanin. Ascorbic acid also has a role to play as an inhibitor tyrosinase. Tyrosinase is essential for the formation of melanin, so if it is inhibited then the action of pigmentation is reduced.

The use of vitamin C in scarring:

The use of vitamin C for scarring of the skin is a recent discovery. In this case the vitamin C has to be delivered in high dosage to the skin and as a result of that more collagen will be formed and normal collagen that is often found tethering scars will be replaced with normal collagen and the scars will fill up to a degree and become less noticeable. This process seems to be best effected with the use of iontophoresis. Enclosed is a photograph to show the numerous effects of vitamin C when iontophoresed onto a patient with severe chicken pox scars. You will notice that after a period of 24 treatments of iontophoresis of vitamin C the skin looks smoother, the pigmentation has been reduced and the scars are flatter.

Skin care has now become scientifically based and is no longer simply hope and magic. As we understand more and more about the physiology of the skin, we also learn how to maintain skin in better health, and as a result, slow down the process of ageing.

Vitamin C has come into the limelight because of its ability to reduce pigmentation and also accelerate collagen formation, but it should always be remembered that vitamin C works best when combined with vitamin E and other anti-oxidants.

Vitamin C has been shown to augment the effects of improving skin elasticity and reduction of pigment formation. Since these essential vitamins are generally extremely sensitive to all-pervasive UV-A light, their daily replacement is an essential for healthy skin. The same is true for the other important skin antioxidants that are sensitive to light such as vitamin E and the carotinoids.

With the knowledge of the physiological changes that occur following exposure of skin to light, one has the ability to create a physiological treatment to counteract those changes. The easiest regime would be to replace the vitamins that have been damaged by exposure to light. What people fail to realise is that replacement of lost vitamins should start soon after the first exposure to the sun in order to maintain as youthful and healthy a skin as possible. Even when photo ageing is advanced, simple daily application of a combination of vitamins C, E, A and beta carotene can make significant changes.

Where photo damage has become established, the replacement of these vitamins can be facilitated with the use of iontophoresis. Up to 400% better penetration (compared to simple topical application) can be achieved with iontophoresis.

Healthy Aging with Vitamin E

Healthy aging is everybody’s goal. Vitamin E is a family affair. Vitamin E is perhaps a family of eight mixtures that includes both gamma-tocopherol and alpha-tocopherol. Vitamin E is a fat soluble vitamin with antioxidant quality that safeguards your cell membranes from the damage caused by free radicals. Free radicals can cause cell injury that may add to the development of heart disease and cancer. Vitamin E is a fat-soluble vitamin and substantial antioxidant that helps protect cells from everyday damage. Vitamin E is also vital for healthy skin and hair. Foods usually rich in vitamin E include nuts, such as almonds, vegetable oils, seeds, wheat germ, spinach and other dark, green leafy vegetables. Vitamins are helpful for one who need them. On the other side, if you have enough vitamins, then you will not need treatment of vitamins. Generating vitamins in your system, which has too much already, or sufficient nutrients can cause harm. Vitamins will make you feel younger as well as make you feel good about yourself, providing you need therapies matches for your system.

People consuming anti-clotting drugs such as Warfarin (Coumadin, Panwarfin) should avoid vitamin E pills. In knowledge, having high doses of Vitamin E may actually make things severe as they may increase the risk for heart disease in those already at risk (people with high blood pressure, cholesterol, and diabetes).

Foods enriched in Vitamin E are broccoli, carrots, kale, grapes, onions, sweet potatoes, blueberries, and oranges. Kale is not be the first thing when making a smoothie, but it’s certainly one of the best one. Dark leafy greens like kale are wrapped with calcium in a form that is easier for the body to absorb than the calcium found in milk. Kale is also one of the some plants which have iron. The phytonutrient bonus incompany with kale also have vitamin C required to ameliorate the absorption of non-heme iron. Successful aging of course comprises social, physical as well as psychological wellbeing.

α-Tocopherol (vitamin E) is one of the most important vitamins for cosmetics. Acting in harmony with ascorbate (vitamin C) vitamin E protects skin cells from oxidative attacks of radicals and peroxides. As tocopherol is easily oxidized and quickly loses the reductive activity, tocopherly acetate, a stable derivative of tocopherol, is popularly formulated. However, its use for aqueous formulations is restricted due to its oily form and poor solubility in water. α-Tocopherol (sodium dl-α-tocopheryl phosphate TPNa) is a water-soluble powdery vitamin E. The oxygen sensitive hydroxyl group of tocopherol is chemically modified and protected with a phosphoryl group. Once absorbed in skin TPNa is readily converted to the active tocopherol via hydrolysis catalyzed by phosphatas, a ubiquitous enzyme in skin.

- High effectiveness to prevent Inflammation after laser treatment, after sun burn after peeling to prevent inflammation, adjust skin roughness, improve redness, pain or soreness at early stage.

- Protect skin cell from attacks of radical and peroxides (for wrinkles, for acne).

- Synergy effects with Vitamin C. We strongly recommend the combination treatment of Vitamin C and Vitamin E for its effectiveness of elimination of free radical.

The topical replacement of light sensitive vitamins C, E, A and beta carotene is essential to maintain skin health. To get even better results, iontophoresis may be used to facilitate the penetration of ionised forms of vitamin E and C.


"Iontophoresis" means a method of treatment to drive a substance having a positive or negative charge into or through tissue/skin. Thus, iontophoresis can be used for dermal and transdermal delivery of a substance, including for diagnostic assays, forensic evaluation, delivery of a substance and the like. Accordingly, the method can be used for delivery of substances for human and veterinary use.

In iontophoresis, two electrodes are placed in contact with the tissue/skin. One electrode is conveniently a pad of absorbent material soaked with the composition to be administered and a voltage is applied between the two electrodes. Usually iontophoresis is preformed with lead electrodes but other types of electrodes known in the art for iontophoresis can be used. The size and shape of the positive electrode usually corresponds to the size and shape of the area to be treated and its thickness and can readily be determined by those of skill in the art. Usually an intermediary material is contacted with the tissue/skin and the in turn is in contact with the electrodes. The intermediary material of suitable size and shape is treated with the composition to be administered usually before being placed in contact with the tissue/skin. The electrode is then placed in contact with the intermediary material. The negative electrode is placed on an optional site of dermis, again usually with an intermediary material between it and the tissue. If the neck or head is treated, iontophoresis is carried out with the current density slowly increased to the peak value desired and stopped when an unpleasant feeling is conveyed. If small electrodes are used or the composition dries out, the intermediary material can be treated periodically with the composition being administered.

VitaminC+E combination
Iontophoresis treatment
at Dr Tu’s Clinic - HCMC

In the iontophoresis method of the invention, the lipid vesicle preferably have a positive or negative surface charge which enhance their penetration as a result of the electrode of the same charge as the iontophoresis unit. Because of the surface charge the penetration can be greatly increased by iontophoresis. The prolonged release rate and the reduced dispersal rate provide a controlled administration rate of the substance at or near the administration site which gives a concentrated and prolonged accuracy of action with less systemic side effects. When administered as a multiphase lipid vesicle composition, the free ionized form of the substance also penetrates better into the tissue because of the presence of the lipid vesicles which hydrate the skin.

The iontophoresis method can be conducted with conventional equipment using conventional pulse magnitude duration of the pulse and frequency of the any cycle. By way of non-limiting example, when the substance is a dye or an anesthetic, the electric current is from about 1 to about 5 milliampere, preferably, from about 1 to about 3 milliampere, and the duration is from about 1 to 15 minutes, preferably from about 5 to about 10 minutes and especially about 5 minutes.

Substances that can be transported into or through the tissue of a subject include any of a wide variety of cosmetic, diagnostic and biologically-active materials which can be encapsulated within a lipid vesicle. Some substances can have different uses depending on the circumstances and dose, for example, Vitamin E can be a nutrient or as an anti-aging antioxidant, some proteins, hormones and enzymes can be pharmaceuticals or diagnostic agents.


Jennifer Lopez

Placenta cosmetics are another proven trend that follows in the heels of ayurveda skincare, skin patches, and acid peels. First started with a simple injection in the 1930s, it has been hailed as an excellent skin healer because of its nourishing qualities. Since the placenta is the lining of the womb, which helps the fetus get its food, placenta cosmetics has been proven to produce wonders for the skin.

Placenta is best known as an anti-aging product. Placenta cosmetics do their jobs by stimulating cell renewal function in the body. Ultimately, cell renewal function helps to slow down the aging process we dread. During the early years of its use, cosmetics manufacturers thought that placenta cells helped to boost the stimulating of tissue growth and removed wrinkles. It achieved a reputation in the past as being strictly for the rich and famous. Reports have surfaced that Jennifer Lopez used this treatment earlier in her career. After all, each injection of placenta costs in the $5.000 to $10.000 range.

Today, placenta use is now a luxury for “normal” people as well. Today, there are plenty of placenta based natural skin care products that include an assortment of wrinkle creams and face masks. Not restricted to women, there are also aftershaves for men, eye creams, breast creams, and collagen based creams that helps moisturize the skin and actually aid with the body’s natural ability to produce collagen for smoother skin. Placenta based cream scrubs also help to clear pores and stimulate blood circulation, which helps prevent cellulite (remember, poor blood circulation and a slow metabolism helps lead to the buildup of cell waste beneath the surface of the skin.)

A common conception within the scientific industry is that natural skin care products, like placenta, do a better job of healing the skin than those without cell extracts. If this is the case, placenta cosmetic products are as natural as you can get – given the fact the placenta originates straight from the womb’s lining and is responsible for nourishing the fetus. It is always important, though, to read the product labels of any cosmetic product you see on the shelves.


Placenta extracts are widely used in cosmetology and dermatocosmetology. The effectiveness of skin treatment with the use of Placenta extracts and Iontophoresis, that increases penetration of placental components into skin, has also been clinically proven. Iontophoresis is a method that combines galvanic current effect with an active preparation. Iontophoresis oxygenates skin and increases components absorbency.

Iontophoresis Therapy

Originally, the technology of ionic permeability was used in medicine for the "guaranteed delivery" of medicinal preparations, such as corticosteroids, different anaesthetics, epinephrine and non-steroidal anti-inflammatory drugs into skin.

When used externally, there are a few ways how medicinal preparations can penetrate into skin: transepidermal way, via sebaceous glands, hair follicle or perspiratory glands. The last one is the most effective. Iontophoresis is an effective way of the guaranteed delivery of water-soluble medicinal preparations deep into epidermis, where excess melanin is localized, and into derma, where synthesis of collagen and elastin takes place.

Outcomes: After a course of treatments with placenta preparations the following results have been achieved:

· decolorizing of pigment spots;

· significant smoothing of wrinkles;

· skin improvement of post acne scarring effect;

· clearly seen skin lifting and face outline conditioning.

It is necessary to point out that the length of treatment period is chosen individually, and the above mentioned procedure can guarantee:

· Excellent results in face contour modelling that could not be reached with the external application of cosmetic and medicamental preparations.

· Skin colour improvement. Besides, the effect was observed at early stages of treatment and thereafter it kept boosting throughout the whole course of treatment.

· Decrease of hyperpigmentation which is proof of effective skin whitening.

· Accelerated wound healing, intensification of blood hemophoresis.

· Anti-inflammatory action.

· Best results in skin revitalising, anti-aging (including photo ageing) and protection from stress.

Placenta extracts produce whitening effect upon skin due to its triple action: they suppress melanin synthesis, take out the existing pigment and oxygenate skin.


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